I have a few things where I want to accomplish early next semester as some next steps. I want to perform an experiment where I would pipe 40% confluent cells into the agarose gel molds like I did previously. Instead of leaving them and trying to pipe out and in media every day, I would pipe out liquid the next day and stick in the PDMS printhead and start flowing media to it in order to keep it alive. If the cells don't get sucked up or impede a good seal, it would feasibly not let the cells flow back up through the waste tube with the media. This would circumvent the problem of cells not sticking together in the wells and just flowing out through the media.
The second place to go involving cells next semester would be tracking cells through the printhead via our microPIV experiments. These are explained in our research abstract. I may actually gear some work towards understanding and performing microPIV experiments and simulations with that part of our group. However, one experiment we want to perform is infusing cells with red or green fluorescence. This would allow our laser to be able to track individual cells or aggregates through the printhead.
- Assay Method for Polymer-Controlled Antibiotic Release
- Comparison of Submerged and Unsubmerged Printing of Ovarian Cancer Cells
- Maximizing Fibroblast Adhesion on Protein-Coated Surfaces Using Microfluidic Cell Printing
- Microfluidic Cell Culture
- Microfluidic Cell Culture Systems for Cellular Analysis
- Optimal Tube Length for the Submerged Printing of Cells
- Versatile, Fully Automated, Microfluidic Cell Culture System